Table 1 DOE evaluation of three cell-culture process parameters
From: Cell-culture growth conditions resulting in the oxidation of a recombinant antigen-binding fragment
Bioreactor | DO% | Temperature (°C) | Supplement at day |
|---|---|---|---|
1 | 50 | 37 | 2,4,6 |
2 | 30 | 37 | 3,5,7 |
3 | 30 | 32 (day 6 shift from 37 to 32) | 3,5,7 |
4 | 50 | 32 (day 6 shift from 37 to 32) | 2,4,6 |
5 | 30 | 37 | 2,4,6 |
6 | 50 | 37 | 3,5,7 |
7 | 30 | 32 (day 6 shift from 37 to 32) | 2,4,6 |
8 | 50 | 32 (day 6 shift from 37 to 32) | 3,5,7 |