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Table 1 DOE evaluation of three cell-culture process parameters

From: Cell-culture growth conditions resulting in the oxidation of a recombinant antigen-binding fragment

Bioreactor DO% Temperature (°C) Supplement at day
1 50 37 2,4,6
2 30 37 3,5,7
3 30 32 (day 6 shift from 37 to 32) 3,5,7
4 50 32 (day 6 shift from 37 to 32) 2,4,6
5 30 37 2,4,6
6 50 37 3,5,7
7 30 32 (day 6 shift from 37 to 32) 2,4,6
8 50 32 (day 6 shift from 37 to 32) 3,5,7