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Table 1 MAO-N variants obtained by directed evolution and respective oxidation rate towards (S)-15 and (R)-15

From: Recent progress in directed evolution of stereoselective monoamine oxidases

MAO-N variant Mutation (S)-15 (%) (R)-15 (%) Time (h)
MAO-N D5 I246M/N336S/M348K/T384N/D385S 0 0 48
MAO-N D9A D5+ F210M/L213C/M242V/I246T 0 0 48
MAO-N D9B D5+ F210L/L213T/M242V/I246T 0 0 48
MAO-N D9C D5+ F210L/L213T/M242Q/I246T 0 0 48
MAO-N D9D D5+ F210M/L213C/M242Q/I246T 0 0 48
MAO-N D10 D5+ W430G 75 26 48
MAO-N D11A D10+ F210M/L213C/M242V/I246T 100 12 30
MAO-N D11B D10+ F210L/L213T/M242V/I246T 100 7 4
MAO-N D11C D10+ F210L/L213T/M242Q/I246T 100 < 1 6
MAO-N D11D D10+ F210M/L213C/M242Q/I246T 100 14 35