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Table 4 Purification strategy employed for xylanase from different microorganisms

From: A detailed overview of xylanases: an emerging biomolecule for current and future prospective

Source organism Purification methodology Specific activity (IU/mg) Fold purification Recovery (%) Km (mg/mL) Vmax (μmol/mg/min) Application of purified enzyme References
Combined purification strategy
 Bacillus sp. SV-34S Ammonium sulfate precipitation (80%) 2803.1 10.62 88.0 3.7 133.33 IU/mL Paper and Pulp industry Mittal et al. (2013)
Carboxymethyl Sephadex C-50 cation exchange chromatography 3417.2 12.94 13.44
 Bacillus sp. GRE7 Ammonium sulfate (40–80%) 191.1 3.9 71 2.23 296.8 IU/mg Eucalyptus kraft pulp bio-bleaching Kiddinamoorthy et al. (2008)
DEAE-Cellulose 582.9 11.9 48
Sephadex G-75 1392.6 28.5 27
 Arthrobacter sp. Ammonium sulfate 162 2 74 0.9 3571 Khandeparkar and Bhosle (2006)
Sephadex G-200 282.6 3.5 62
DEAE-Sepharose FF 444.2 5.5 49
CM-Sepharose FF 1697.7 21 14
 Thermotoga thermarum Ni-affinity chromatography 192 6.9 82.3 1.8 769 XOS generation Shi et al. (2014)
 Glaeocola mesophila KMM 241 Ammonium sulfate
Dialysis
Ni+2-NTA Agarose resins
77 9.1 17.4 1.22 98.31 XOS generation, Sea and saline food processing, bakery industries Guo et al. (2009)
 Streptomyces olivaceus (MSU3) Ammonium sulfate fractionation 37.35 ± 1.33 1.045 ± 0.002 60.69 ± 2.38 8.16 250.01 High sugar yield and bioethanol generation ability using lignocellulosic biomass Sanjivkumar et al. (2017)
Dialysis 82.40 ± 2.50 2.298 ± 0.021 38.29 ± 1.53
DEAE–cellulose column 116.98 ± 2.28 3.260 ± 0.024 28.15 ± 1.87
Sephadex-G-75 column 153.11 ± 2.11 4.270 ± 0.026 15.57 ± 0.85
 Penicillium glabrum Ammonium sulfate fractionation 291.78 3.25 84.19 1.2–5.3 212.10–393.17 Purified enzyme suitable for animal feed additives, clarification, and maceration of juices and wines Knob et al. (2013)
Molecular exclusion chromatography (Sephadex G-75 column) 457.89 5.10 76.92
 Trichoderma inhamatum Dialysis followed by ion exchange chromatography (DEAE Sephadex A-50 column) Xyl I-1257.7
Xyl II-
1216.4
Xyl I, II-1.9 Xyl I-62.7
Xyl II-3.7
Xyl I-1.6–14.5
Xyl II-4.0–10.7
Xyl I-462.2–2680.2 U/mg
Xyl II-1972.7–4553.7 U/mg
XOS generation da Silva et al. (2015)
 Chrysoporthe cubensis Q Sepharose anion exchange column followed by Sephacryl S-200 gel filtration column P2-1.2
P3-1.4
P2-13.4
P3-1.3
P2-1.81
P3-1.18
Efficient saccharification of sugarcane bagasse de Sousa et al. (2017)
 Myceliophthora heterothallica F.2.1.4 Ni Sepharose® resin containing HisTrap HP column 102.2 44 7 13.4 ± 1.5 274.5 ± 1.0 Pulp bleaching de Amo et al. (2019)
 Aspergillus flavus Ammonium sulfate fractionation (40–60%) 621.4 2.7 46.7 Efficiently hydrolyzed pretreated corncobs for generation of XOS Chen et al. (2019)
Gel filtration (HiPrep 16/60 Sephacryl S-100 HR column) chromatography 838.2 3.7 34.8
 Penicillium chrysogenum Ion exchange chromatography (CM Sephadex C-50 column) 438.3 9.8 49.3 2.3 mM 731.8 U/mg Beverage, bakery, and feed industry. For production of xylooligosaccharides and bioethanol Terrone et al. (2018)
Size exclusion chromatography (Sephadex G-100 column) 834.2 18.7 31.1
Single step purification strategy
 Streptomyces P12-137 Ammonium sulfate precipitation followed by dialysis F5-45.44
F6-36.48
4.18 F5-60.63
F6-73.02
F5-0.2012
F6-0.0388
F5-0.4742 s−1
F6-0.6314 s−1
Efficient XOS generation Coman et al. (2013)
 Sorangium cellulosum So9733-1 Ni-affinity followed by dialysis and concentration 4.11 4.03 43.84 38.13 10.69 Hydrolysis of xylan rich substrates and food industries Wang et al. (2012)
 Aspergillus tamarii Kita Single step purification carboxymethyl-cellulose (CM-cellulose) chromatography 1215.89 7.43 36.72 7.59–8.13 1178.56–1330.20 XOS generation Heinen et al. (2018)
 Scytalidium thermophilum ATCC No. 16454 Ammonium sulfate precipitation (50%) 125.9 2.5 52 2.4 168.6 IU/mL Agricultural biomass hydrolysis for application in bioethanol refinery Kocabacs et al. (2015)
ATPS (7% Triton X-114 top phase) 79.8 2.7 79
10 kDa molecular weight cut-off (MWCO) 841.1 4.3 25
 Aspergillus oryzae LC1 Ammonium sulfate
Precipitation followed by dialysis
631 4.1 21 0.2 172.2 XOS generation Bhardwaj et al. (2019)
Anion exchange Chromatography (DEAE-Sephadex A-50) 469 3 31.9
Gel Filtration Chromatography (Sepharose G-100) 101.5 6.6 78.7
ATPS with Triton X-114 (5% v/v) 508.9 3.3 69.4
ATPS with PEG (22.5% MgSO4 Salt 11.3% PEG 8000) 2004.3 13 86.8