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Table 2 Plackett–Burman for acid-pretreated substrate

From: Citrus limetta peels: a promising substrate for the production of multienzyme preparation from a yeast consortium

Run orderExperimental parametersEnzyme production (IU mL−1)a
Temperature (°C)pHMoistening agentIncubation time (h)Inoculum size (mL g−1)Moisture content (%)PretreatmentEGBGLXylPECAMY
1307MSM480.2580Acid5.628.758.8512.243.09
2357YEP480.2550Acid7.4011.8714.583.806.09
3355YEP720.2580Nil9.2214.8511.1226.4617.86
4305MSM720.580Acid3.956.0917.0227.0914.07
5357MSM480.550Acid12.5420.257.1018.419.79
6357YEP720.2550Nil9.3718.5011.3615.6712.97
7307YEP720.580Nil10.9515.5912.0118.6417.02
8307YEP480.580Acid11.5613.513.5010.466.65
9355YEP480.2580Acid12.5410.833.8310.942.26
10357MSM720.2580Acid11.7511.9918.2015.928.56
11355YEP720.550Acid16.4815.5425.136.8425.47
12357MSM720.580Nil9.6612.628.8825.5613.42
13305YEP720.550Acid12.5715.0024.6711.2121.37
14355MSM480.580Nil7.658.3314.2614.5917.66
15305MSM720.2550Acid011.7216.605.6414.94
16355MSM480.550Nil11.8412.4814.658.8315.58
17307MSM720.2550Nil5.1812.467.3914.399.72
18307YEP480.550Nil14.7512.9317.2214.0916.57
19305YEP480.2580Nil12.6213.6718.0614.1016.28
20305MSM480.2550Nil6.018.5315.0910.4013.42
  1. SSF of acid-pretreated Citrus limetta was carried out by yeast consortium, factors affecting multienzyme PREPARATION were adjusted at two levels (temperature 30, 35 °C; pH 5, 7; Moistening agent mineral salt medium or MSM, yeast extract and peptone; incubation time 48, 72 h; inoculum size 0.25, 0.5 mL g−1; moisture content 50, 80%; pretreatment acid, no treatment, or nil), and the production of endoglucanase (EG), β-glucosidase (BGL), xylanase (Xyl), pectinase (PEC), and amylase (AMY) was taken as response
  2. aThe values represent average of triplicate with insignificant standard deviation