Bioresources and Bioprocessing

Table 2 Plackett–Burman for acid-pretreated substrate

From: Citrus limetta peels: a promising substrate for the production of multienzyme preparation from a yeast consortium

Run order	Experimental parameters							Enzyme production (IU mL⁻¹)^a
Run order	Temperature (°C)	pH	Moistening agent	Incubation time (h)	Inoculum size (mL g⁻¹)	Moisture content (%)	Pretreatment	EG	BGL	Xyl	PEC	AMY
1	30	7	MSM	48	0.25	80	Acid	5.62	8.75	8.85	12.24	3.09
2	35	7	YEP	48	0.25	50	Acid	7.40	11.87	14.58	3.80	6.09
3	35	5	YEP	72	0.25	80	Nil	9.22	14.85	11.12	26.46	17.86
4	30	5	MSM	72	0.5	80	Acid	3.95	6.09	17.02	27.09	14.07
5	35	7	MSM	48	0.5	50	Acid	12.54	20.25	7.10	18.41	9.79
6	35	7	YEP	72	0.25	50	Nil	9.37	18.50	11.36	15.67	12.97
7	30	7	YEP	72	0.5	80	Nil	10.95	15.59	12.01	18.64	17.02
8	30	7	YEP	48	0.5	80	Acid	11.56	13.51	3.50	10.46	6.65
9	35	5	YEP	48	0.25	80	Acid	12.54	10.83	3.83	10.94	2.26
10	35	7	MSM	72	0.25	80	Acid	11.75	11.99	18.20	15.92	8.56
11	35	5	YEP	72	0.5	50	Acid	16.48	15.54	25.13	6.84	25.47
12	35	7	MSM	72	0.5	80	Nil	9.66	12.62	8.88	25.56	13.42
13	30	5	YEP	72	0.5	50	Acid	12.57	15.00	24.67	11.21	21.37
14	35	5	MSM	48	0.5	80	Nil	7.65	8.33	14.26	14.59	17.66
15	30	5	MSM	72	0.25	50	Acid	0	11.72	16.60	5.64	14.94
16	35	5	MSM	48	0.5	50	Nil	11.84	12.48	14.65	8.83	15.58
17	30	7	MSM	72	0.25	50	Nil	5.18	12.46	7.39	14.39	9.72
18	30	7	YEP	48	0.5	50	Nil	14.75	12.93	17.22	14.09	16.57
19	30	5	YEP	48	0.25	80	Nil	12.62	13.67	18.06	14.10	16.28
20	30	5	MSM	48	0.25	50	Nil	6.01	8.53	15.09	10.40	13.42

SSF of acid-pretreated Citrus limetta was carried out by yeast consortium, factors affecting multienzyme PREPARATION were adjusted at two levels (temperature 30, 35 °C; pH 5, 7; Moistening agent mineral salt medium or MSM, yeast extract and peptone; incubation time 48, 72 h; inoculum size 0.25, 0.5 mL g⁻¹; moisture content 50, 80%; pretreatment acid, no treatment, or nil), and the production of endoglucanase (EG), β-glucosidase (BGL), xylanase (Xyl), pectinase (PEC), and amylase (AMY) was taken as response
^aThe values represent average of triplicate with insignificant standard deviation

Back to article page