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Table 4 Box–Behnken design matrix for production of endoglucanase (EG), β-glucosidase (BGL), xylanase (Xyl), pectinase (PEC), and amylase (AMY) under SSF of Citrus limetta peels (CLP) by the yeast consortium

From: Citrus limetta peels: a promising substrate for the production of multienzyme preparation from a yeast consortium

Run orderExperimental parametersEnzyme production (IU mL−1)a
Alkali (%)Incubation time (h)pHMoistening agentEGBGLXYLAMYPEC
10.5486MSM + YE8.8610.3712.387.1231.34
21486MSM + YE1.975.545.986.6531.23
30.5966MSM + YE8.7216.512.517.1830.42
41966MSM + YE4.365.428.696.2822.64
50.75725MSM3.642.855.364.4121.10
60.75727MSM4.123.436.677.9726.39
70.75725YEP3.762.045.353.8921.94
80.75727YEP5.9614.655.510.5630.73
90.5726MSM5.713.1215.6714.0229.42
101726MSM1.243.846.419.9226.49
110.5726YEP6.4015.625.5610.2634.39
121726YEP2.3712.9110.749.7432.6
130.75485MSM + YE2.820.8887.092.2625.41
140.75965MSM + YE4.4674.243.560.9224.79
150.75487MSM + YE4.541.854.01.5724.88
160.75967MSM + YE6.686.066.226.2228.92
170.5725MSM + YE13.309.0913.7112.9423.19
181725MSM + YE0.929.569.143.6730.77
190.5727MSM + YE7.5715.7111.1511.6927.51
201727MSM + YE6.534.176.2810.1427.51
210.75486MSM2.093.874.561.6522.64
220.75966MSM4.152.775.643.2924.53
230.75486YEP2.423.245.864.1320.61
240.75966YEP4.987.124.974.0430.57
250.75726MSM + YE2.916.786.337.9632.07
260.75726MSM + YE5.342.786.406.8228.34
270.75726MSM + YE3.378.277.997.1127.42
  1. Alkali pretreatment to CLP was given using 0.5, 0.75 or 1% NaOH, incubation time varied 48, 72, or 96 h, pH adjusted to 5, 6, or 7, and moistening agent was used mineral salt medium (MSM) or yeast extract (YE) or both (MSM + YE)
  2. aThe values represent average of triplicate with insignificant standard deviation