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Bioresources and Bioprocessing

Table 2 Fluorinase variants obtained by directed evolution
figure a

From: Enzyme-catalyzed C–F bond formation and cleavage

Fluorinase

T (oC)

RCC (analytical) (%)

RCCa (overall) (%)

FIA1

42

8 ± 1

7 ± 1

FIA1

47

11 ± 2

8 ± 2

fah2081 (A279Y)

42

32 ± 3

24 ± 2

fah2114 (F213Y, A279L)

47

46 ± 2

34 ± 3

  1. aOverall RCC = % radioactivity in supernatant W% RCC based on radioHPLC. Typical % radioactivity in supernatant is in the range 73–80%
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