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Fig. 4 | Bioresources and Bioprocessing

Fig. 4

From: Metabolic pathway analysis for in silico design of efficient autotrophic production of advanced biofuels

Fig. 4

Multiple gene deletion simulation by elementary mode analysis. Single (a, d), double (b, e) and triple (c, f) gene deletion simulation on the recombinant strains for isobutanol and hexadecanol synthesis were implemented to identify combination of deleted genes that eliminate inefficient pathways for product synthesis, while keeping efficient ones hence redirecting the cell’s phenotypic space towards maximal product yield. Three criteria for identifying target genes are (1) product yields (maximal and mean YP g/g, yellow triangle, orange diamond), (2) fractional pathways for product synthesis (P-frac %, green square) and (3) biomass yield (mean YX g/g, empty circle) after each gene deletion simulation. Mean value is an average of minimal and maximal values. Target genes for deletion (indicated by red arrow) would reshape phenotypic space by pushing towards high product yield (max P-frac, max YP), while retain cell viability (YX > 0)

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