Fig. 5

Phenotypic space of C. necator H16 mutants designed by elementary mode analysis for high yield of isobutanol (a) and hexadecanol (b). Each black circle represents individual pathways in which a linear combination of all pathways forms possible phenotypes existing in the designed mutant with yields shown in colored surface plot. The x and y axes are uptake rate for H₂ and CO₂ of each pathway relative to its total gas uptake. Dashed lines show phenotypic space of the designed mutant under controlled gas input composition at fractional H₂ input (f_qH2) > 0.67. After the in silico multiple gene deletions, predicted yield space (presented as colored surface plot) was higher compared to that by the wild-type in Fig. 3