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Table 1 PHA content and composition produced by the Methylosinus-dominated culture

From: The effect of methane and odd-chain fatty acids on 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) synthesis by a Methylosinus-dominated mixed culture

Odd-chain fatty acids

Methane availability

Code

PHA content (wt%)

3HV fraction (mol%)

Total suspended solid (TSS) (g TSS L−1)

None

Yes

M1

8.6

0.0

0.42 ± 0.01

No

M0

1.3

0.0

0.35 ± 0.01

Propionic acid

Yes

M1P

3.5

22.6

0.37 ± 0.01

No

M0P

2.8

20.5

0.36 ± 0.00

Valeric acid

Yes

M1V

14.1

65.0

0.47 ± 0.00

No

M0V

8.5

56.4

0.45 ± 0.01

Odd-chain fatty acids

Methane availability

Code

CH4 consumed (mmol CH4 g TSS−1 day−1)

CO2 produced (mmol CO2 g TSS−1 day−1)

C3 consumed (mmol C3 g TSS−1)

C5 consumed (mmol C5 g TSS−1)

None

Yes

M1

7.1 ± 1.2

2.4 ± 0.3

–

–

No

M0

–

N/A

–

–

Propionic acid

Yes

M1P

5.2 ± 0.3

1.6 ± 0.3

2.1

–

No

M0P

–

N/A

1.6

–

Valeric acid

Yes

M1V

4.3 ± 0.4

0.8 ± 0.2

–

2.2

No

M0V

–

N/A

–

2.3

  1. The content and composition of PHA was measured after 72 h of incubation in the presence and absence of methane with supplementation of odd-chain fatty acids
  2. N/A not applicable