Fig. 2
From: Selection and screening strategies in directed evolution to improve protein stability
Screening and selection methods based on protein stability biosensors. Diversified genetic libraries can be handily screened or selected utilizing various protein stability biosensors. These biosensors establish direct linkages between the folding abilities of POIs and easily detectable phenotypes of reporter proteins, such as antibiotic resistance markers and fluorescent proteins. The stable or soluble mutants can be identified by screening the cells for high fluorescence or selecting the cells with elevating concentrations of antibiotics