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Fig. 5 | Bioresources and Bioprocessing

Fig. 5

From: Bacterial cytochrome P450-catalyzed regio- and stereoselective steroid hydroxylation enabled by directed evolution and rational design

Fig. 5

Relative amounts of hydroxylated products of testosterone (a) catalyzed by P450BM3 and its mutants KSA-1 (F87A/A330W) and KSA-9 (A82N/F87A); and hydroxylated products of progesterone (b) catalyzed by P450BM3 and its mutants KSA-1 (F87A/A330W) and KSA-14 (A82N/F87A), respectively. Reaction conditions: 1 mM substrate in KPi buffer pH 7.8, 24 h, 25 °C, resting cells containing expressed P450 mutants

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