Fig. 6From: Bacterial cytochrome P450-catalyzed regio- and stereoselective steroid hydroxylation enabled by directed evolution and rational designMutants of P450BM3 from Commandeur’s group. The shifts of selectivity of hydroxylated products of testosterone catalyzed by M01, M11 and their mutants M01-A82W, M11-A82W, M01-A82W/S72V. The major hydroxylated products of testosterone by M01 and M11 are shown in a and by their mutants are showed in b. Reaction conditions: 500 nM purified enzymes containing 0.5 mM testosterone in 250 µL 100 mM KPi buffer pH 7.4, 25 µL NADPH regeneration system containing 2 mM NADPH, 3 mM glucose-6-phosphate and 4 U/mL of glucose-6-phosphate dehydrogenase, 1 h, 24 °CBack to article page