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Fig. 6 | Bioresources and Bioprocessing

Fig. 6

From: Bacterial cytochrome P450-catalyzed regio- and stereoselective steroid hydroxylation enabled by directed evolution and rational design

Fig. 6

Mutants of P450BM3 from Commandeur’s group. The shifts of selectivity of hydroxylated products of testosterone catalyzed by M01, M11 and their mutants M01-A82W, M11-A82W, M01-A82W/S72V. The major hydroxylated products of testosterone by M01 and M11 are shown in a and by their mutants are showed in b. Reaction conditions: 500 nM purified enzymes containing 0.5 mM testosterone in 250 µL 100 mM KPi buffer pH 7.4, 25 µL NADPH regeneration system containing 2 mM NADPH, 3 mM glucose-6-phosphate and 4 U/mL of glucose-6-phosphate dehydrogenase, 1 h, 24 °C

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