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Fig. 7 | Bioresources and Bioprocessing

Fig. 7

From: Bacterial cytochrome P450-catalyzed regio- and stereoselective steroid hydroxylation enabled by directed evolution and rational design

Fig. 7

Controlling regio- and diastereoselectivity at position 16 of various steroids. a The directed evolution strategy of P450BM3: mutability landscapes (MLs), molecular dynamics simulation (MDS) and iterative saturation mutagenesis (ISM) are combined to design the mutations. b Conversion and selectivity of hydroxylated products of testosterone catalyzed by the best two P450 BM3 mutants WIFI-WC (R47W/S72I/A82F/S72I-Y51W/L181C) and WWV-QRS (R47W/A82W/F87V-L181Q/T436R/M177S), respectively. Reaction conditions: 1 mM testosterone in 600 μL, 100 mM KPi buffer pH 8.0 for 24 h, 220 rpm, 37 °C

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