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Bioresources and Bioprocessing
Fig. 3 | Bioresources and Bioprocessing

Fig. 3

From: Development of an intensified fed-batch production platform with doubled titers using N-1 perfusion seed for cell culture manufacturing

Fig. 3

a Viable cell density (VCD) and b cell viability profiles for the enriched N-1 seed at 200-L scale (n = 5) for Process B and the perfusion N-1 seed at lab scales (n = 3) for Process C; c VCD; d cell viability, and e normalized titer profiles for the subsequent fed-batch cell culture performance using Process B in 1000-L bioreactors (n = 5), Process B in 5-L satellite bioreactors (n = 4) using the same N-1 seed as 1000-L and Process C in 5-L bioreactors (n = 4) for mAb2 production. The titer on Day 10 for Process C at 5-L scale was normalized as 1 for mAb2. Values are reported as average ± standard deviation (n ≥ 3)

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