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Bioresources and Bioprocessing
Fig. 5 | Bioresources and Bioprocessing

Fig. 5

From: Development of an intensified fed-batch production platform with doubled titers using N-1 perfusion seed for cell culture manufacturing

Fig. 5

a Viable cell density (VCD) and b cell viability profiles for the batch N-1 seed at 200-L scale (n = 5) for Process A, the perfusion N-1 seed at 5-L scale (n = 3), and the perfusion N-1 seed at 200-L scale (n = 1) for Process C; c VCD, d cell viability, e normalized titer profiles, and f in-process quality attribute profiles at harvest for the subsequent fed-batch cell culture performance using Process A in 1000-L bioreactors (n = 5), and Process C in 5-L bioreactors (n = 4) and 500-L bioreactor (n = 1) for mAb4 production. The maximum titer on Day 12 for Process C at 500-L scale was normalized as 1 for mAb4. All Process C values at large scale are n = 1, Process A quality attributes are reported as average ± difference/2 (n = 2, because only two Day 14 in-process samples were available), and all other values are reported as average ± standard deviation (n = 3–5)

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