Table 1 Medium nutrient concentrations used in Process A, Process B and Process C
Medium type | mAb/process | Media name | Total amino acids (mM) | Glucose (g/L) | Osmolality (mOsm/kg) |
|---|---|---|---|---|---|
Basal | mAb1/Process A, mAb4/Process A, mAb3/Process B | B1 | 69 | 6 | 300 |
mAb1/Process B | B1 enriched1 | 99 | 10 | 380 | |
mAb2/Process B | B1 enriched2 | 118 | 11 | 410 | |
mAb3/Process C | B2 | 103 | 6 | 310 | |
mAb4/Process C | B2 enriched1 | 122 | 10 | 370 | |
mAb1/Process C, mAb2/Process C | B2 enriched2 | 137 | 10 | 425 | |
Feed | mAb1/Process A, mAb4/Process A | F1 | 344 | 60 | 1310 |
mAb1/Process B | 1.3 × F1 | 404 | 78 | 1450 | |
mAb2/Process B | 1.3 × F1 enriched1 | 446 | 78 | 1575 | |
mAb3/Process B | 1.3 × F1 enriched2 | 513 | 80 | 1810 | |
mAb1/Process C | F2 | 404 | 110 | 1570 | |
mAb3/Process C, mAb4/Process C | F2 enriched1 | 500 | 120 | 2000 | |
mAb2/Process C | 1.3 × F2 enriched2 | 650 | 156 | 2100 |