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Table 3 Changes in Process C for different mAb production

From: Development of an intensified fed-batch production platform with doubled titers using N-1 perfusion seed for cell culture manufacturing

mAbmAb1mAb2mAb3mAb4
ProcessBCBCBCAC
N-2 seedBatch in 50-L wavePerfusion in 50-L waveBatch in 50-L waveShake flasksBatch in 50-L wavePerfusion in 50-L waveBatch in 50-L wavePerfusion in 50-L wave
N-1 seedBatch in 200-LPerfusion in 200-LBatch in 200-LPerfusion in 20-LBatch in 200-LPerfusion in 200-LBatch in 200-LPerfusion in 200-L
Fed-batch production1000-L500-L1000-L5-L1000-L500-L1000-L500-L
SD (× 106 cells/mL)3.0163.0154.5101.515
Feed rateFixed at 3.1% of initial volume from Day 4 (D4)Fixed at 4.1% of initial volume from D2Fixed at 3.5% of current volume from D3Fixed at 4% from current volume D12.6% D2–3; 3.5% D4–8; 2.46% D9–13 of initial volume2.6% D1; 3.54% D2–13; initial volumeFixed at 3.6% of initial volume from D3Fixed at 4.5% of initial volume from D1
Temperature shift to32 °C on D532 °C on D334 °C on D634 °C on D4NANANA34 °C on D4