Parameters | Conditions | |||||||
---|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 6 | 7 | ||
Inoculum | Inoculation seeding density (SD) (× 106 cells/mL) | 1.5 | 3 | 11 | 19 | 3 | 11 | 19 |
Media | Basal | Process A (B1) | Process B (B1 enriched1) | Process C (B2 enriched2) | ||||
Feed | Process A (F1) | Process B (1.3 × F1) | Process C (F2) | |||||
Feeding strategy | Daily feed rate | 3.6% of initial volume | ||||||
Feed start day | 3 | 4 | 2 | 1 | 4 | 2 | 1 | |
Bioreactor condition | Temperature shift to 32 °C | NA | Day 5 | Day 4 | Day 3 | Day 5 | Day 4 | Day 3 |