Table 6 Normalized titers on Day 14 for mAb2 production using different N-1 seed cultures, production inoculation cell densities (SD) and media in 5-L fed-batch bioreactors
Condition | 1 (n = 4) | 2 (n = 2) | 3 (n = 2) | 4 (n = 1) | 5 (n = 2) | 6 (n = 2) |
|---|---|---|---|---|---|---|
N-1 mode | Perfusion | Perfusion | Perfusion | Perfusion | Enriched batch | Enriched batch |
SD (× 106 cells/mL) | 15 | 15 | 15 | 3 | 3 | 3 |
Basal | B2 enriched2 | B2 enriched2 | B1 enriched2 | B2 enriched2 | B2 enriched2 | B1 enriched2 |
Feed | 1.3 × F2 enriched2 | F2 | 1.3 × F1 enriched1 | F2 | F2 | 1.3 × F1 enriched1 |
Titer (normalized weight/L) | 1.19 ± 0.02 | 0.8 ± 0.01 | 0.78 ± 0.03 | 0.24 | 0.39 ± 0.07 | 0.64 ± 0.01 |