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Table 6 Normalized titers on Day 14 for mAb2 production using different N-1 seed cultures, production inoculation cell densities (SD) and media in 5-L fed-batch bioreactors

From: Development of an intensified fed-batch production platform with doubled titers using N-1 perfusion seed for cell culture manufacturing

Condition1 (n = 4)2 (n = 2)3 (n = 2)4 (n = 1)5 (n = 2)6 (n = 2)
N-1 modePerfusionPerfusionPerfusionPerfusionEnriched batchEnriched batch
SD (× 106 cells/mL)151515333
BasalB2 enriched2B2 enriched2B1 enriched2B2 enriched2B2 enriched2B1 enriched2
Feed1.3 × F2 enriched2F21.3 × F1 enriched1F2F21.3 × F1 enriched1
Titer (normalized weight/L)1.19 ± 0.020.8 ± 0.010.78 ± 0.030.240.39 ± 0.070.64 ± 0.01
  1. The Process C titer on Day 10 was normalized as 1 for mAb2