Table 2 The specific activity (U/mg) of GsDI wild type and the mutants at different pHs
From: Engineering a diaphorase via directed evolution for enzymatic biofuel cell application
Enzymes | New mutations based on wild type | pH 5.4 | pH 4.5 |
|---|---|---|---|
Wild type | – | 0.25 ± 0.04 | 0.09 ± 0.01 |
M 1–8 | H37Q, S73T, F105L | 0.44 ± 0.01 | 0.26 ± 0.04 |
M 2–12 | H37Q, S73T, F105L, S68T | 0.80 ± 0.06 | 0.44 ± 0.06 |
M 3–8 | H37Q, S73T, F105L, S68T, G61S, D74V | 0.96 ± 0.12 | 0.61 ± 0.10 |