Table 4 Enzyme kinetics for GsDI wild type and mutants F105L and F105A at pH 5.4 or pH 4.5
From: Engineering a diaphorase via directed evolution for enzymatic biofuel cell application
Enzyme | pH 5.4 | pH 4.5 | ||||
|---|---|---|---|---|---|---|
Km (mM) | kcat (s−1) | kcat/Km (mM−1 s−1) | Km (mM) | kcat (s−1) | kcat/Km (mM−1 s−1) | |
Wild type | 0.59 ± 0.12 | 0.18 ± 0.04 | 0.31 ± 0.05 | 1.36 ± 0.21 | 0.05 ± 0.01 | 0.04 ± 0.01 |
F105L | 0.31 ± 0.07 | 0.24 ± 0.03 | 0.77 ± 0.08 | 0.78 ± 0.17 | 0.13 ± 0.02 | 0.17 ± 0.02 |
F105A | 0.29 ± 0.05 | 0.26 ± 0.05 | 0.89 ± 0.12 | 0.66 ± 0.13 | 0.14 ± 0.02 | 0.21 ± 0.02 |