Table 2 General characteristics of SL4 and SL5 metagenomes
| Â | SL4 | SL5 |
|---|---|---|
1. Pre-processing | ||
 a. Raw reads | ||
  Reads | 73,402 | 46,294 |
  Total bases (bp) | 21,042,303 | 12,428,339 |
  Mean read length (bp) | 286.67 ± 59.44 | 268.47 ± 86.22 |
  Mean GC content (%) | 55.08 ± 12.49 | 54.20 ± 10.61 |
 b. Quality trimming | ||
  Trimmed reads |  |  |
  Reads | 69,514 (94.70%) | 40,658 (87.83%) |
  Total bases (bp) | 20,902,030 (99.33%) | 12,216,171 (98.29%) |
  Mean read length (bp) | 300.69 ± 4.38 | 300.46 ± 7.23 |
  Mean GC content (%) | 57.49 ± 4.94 | 55.70 ± 4.49 |
  Paired reads | 69,494 (99.97%) | 40,604 (99.87%) |
  Paired total bases | 20,896,965 (99.98%) | 12,200,818 (99.87%) |
  Unpaired reads | 20 (0.03%) | 54 (0.13%) |
  Unpaired total bases | 5065 (0.02%) | 15,353 (0.13%) |
2. Assembly and annotation | ||
 a. De novo assembly by idba_ud | ||
  Number of contigs | 117 | 76 |
  N50 (bp) | 420 | 424 |
  Max contig size (bp) | 458 | 462 |
  Min contig size (bp) | 255 | 270 |
  Total assembly size (bp) | 47,020 | 30,607 |
 b. Assembly validation by read mapping | ||
  Number of mapped reads | 40,629 | 23,795 |
  % of total reads | 58.45% | 58.52% |
  Number of unmapped reads | 28,885 | 16,863 |
  % of total reads | 41.55% | 41.48% |
  Average fold coverage | 214.34 X | 204.18 X |