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Table 2 List of the screened mutants from directed evolution using high-throughput screening method of GSH assay

From: A method for high-throughput screening hydrolase of lignin β-aryl ether linkage from directed evolution by glutathione (GSH) assay

Mutants

Mutated site

AAa in the original LigF

AA in the mutant

Activityb

B7

10

P

L

C1

29

E

G

+

91

F

S

C3

23

E

G

E1

99

W

R

236

I

V

F1

145

W

G

F6

125

Q

H

+

H11

109

W

R

AA4

87

P

S

+

AE4

123

I

V

AF1

181

K

E

AH8

83

P

S

155

G

D

BE1

10

P

R

56

A

T

BG5

129

D

G

+

BG11

72

E

G

  1. a AA indicates the abbreviation of amino acid. b“+” indicates that the mutant activity was higher than that of the original LigF; “−” indicates that the mutant activity was lower than that of the original LigF