Skip to main content
Fig. 3 | Bioresources and Bioprocessing

Fig. 3

From: Fungal statin pump protein improves monacolin J efflux and regulates its production in Komagataella phaffii

Fig. 3

TapA function analysis by MJ distribution of various constructed strains. Dry cell weight of GS-PGAP-TapA and wild-type GS115 fed with MJ of 0.3 mmol/L (a), 0.6 mmol/L (b) and 2.3 mmol/L (c) in shake flask. Extracellular and intracellular MJ levels of GS-PGAP-TapA and GS115 fed with MJ of 0.3 mmol/L (d), 0.6 mmol/L (e) and 2.3 mmol/L (f) in shake flask. Dry cell weight (g), extracellular and intracellular MJ (h) and intracellular MJ percentage (i) of J#9-TapA and J#9 in shake flask. Three replicates were tested and experiments were conducted three times. Statistical significance was analyzed for each comparative group at various time points. *P < 0.05 for cell growth of GS115 relative to GS115 with MJ feeding. #P < 0.05 for cell growth of GS-PGAP-TapA relative to GS-PGAP-TapA with MJ feeding. ×P < 0.05 for intracellular MJ of GS115 relative to that of GS-PGAP-TapA. P < 0.05 for extracellular MJ of J#9 relative to that of J#9-TapA. P < 0.05 for intracellular percentage of MJ of J#9 relative to that of J#9-TapA. Data with not significant differences were not marked in figure. Error bars smaller than the plot symbols were not displayed by the GraphPad software

Back to article page