Fig. 5

The conformation of two T. fusca cutinase variants I213A and L90A after MD simulations. The oligoester C₂₄H₄₂O₈ bound complex structures of I213A (a) and L90A (b) compared with WT. Four loops, including loop 58–64, loop 84–92, loop 174–182 and loop 204–216 are shown as cartoons and sticks in I213A (green), L90A (cyan) and WT (grey). The substrate C₂₄H₄₂O₈ is shown as black balls-and-sticks. c The RMSF of these four loops of T. fusca cutinase variants for WT(black), I213A (green) and L90A (cyan) without substrate during the MD simulations