Table 3 Experimental planning matrix (real and coded values) and relative activity results (%) for the conditions evaluated in the reaction system ultrasonic probe with enzymatic extracts produced from swine hair pretreated by ultrasonic probe and without pretreatment
Assays | Ultrasonic probe | Relative activity (%) | |||
|---|---|---|---|---|---|
Time (min) | Power (%) | Pulse | Pretreatment by ultrasonic probe | Without pretreatment | |
1 | −1 (2) | −1 (40) | −1 (1) | 11.3 | 38.4 |
2 | +1 (10) | −1 (40) | −1 (1) | 0.0 | 1.4 |
3 | −1 (2) | +1 (80) | −1 (1) | 2.7 | 16.3 |
4 | +1 (10) | +1 (80) | −1 (1) | 0.0 | 9.1 |
5 | −1 (2) | −1 (40) | +1 (3) | 0.0 | 21.1 |
6 | +1 (10) | −1 (40) | +1 (3) | 0.0 | 0.0 |
7 | −1 (2) | +1 (80) | +1 (3) | 23.3 | 25.2 |
8 | +1 (10) | +1 (80) | +1 (3) | 0.0 | 10.3 |
9 | 0 (6) | 0 (60) | 0 (2) | 0.0 | 0.0 |
10 | 0 (6) | 0 (60) | 0 (2) | 0.0 | 3.0 |
11 | 0 (6) | 0 (60) | 0 (2) | 0.0 | 0.0 |
12 | 0 (6) | 0 (60) | 0 (2) | 0.0 | 0.0 |
Enzymatic extract obtained after FS without exposure to reaction systems | 75.0 U mL−1 | 124.3 U mL−1 | |||