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Table 3 Experimental planning matrix (real and coded values) and relative activity results (%) for the conditions evaluated in the reaction system ultrasonic probe with enzymatic extracts produced from swine hair pretreated by ultrasonic probe and without pretreatment

From: Reactional ultrasonic systems and microwave irradiation for pretreatment of agro-industrial waste to increase enzymatic activity

Assays Ultrasonic probe Relative activity (%)
Time (min) Power (%) Pulse Pretreatment by ultrasonic probe Without pretreatment
1 −1 (2) −1 (40) −1 (1) 11.3 38.4
2 +1 (10) −1 (40) −1 (1) 0.0 1.4
3 −1 (2) +1 (80) −1 (1) 2.7 16.3
4 +1 (10) +1 (80) −1 (1) 0.0 9.1
5 −1 (2) −1 (40) +1 (3) 0.0 21.1
6 +1 (10) −1 (40) +1 (3) 0.0 0.0
7 −1 (2) +1 (80) +1 (3) 23.3 25.2
8 +1 (10) +1 (80) +1 (3) 0.0 10.3
9 0 (6) 0 (60) 0 (2) 0.0 0.0
10 0 (6) 0 (60) 0 (2) 0.0 3.0
11 0 (6) 0 (60) 0 (2) 0.0 0.0
12 0 (6) 0 (60) 0 (2) 0.0 0.0
Enzymatic extract obtained after FS without exposure to reaction systems 75.0 U mL−1 124.3 U mL−1