Assays | Ultrasonic Bath | Relative activity (%) | |||
---|---|---|---|---|---|
Temperature (°C) | Power (%) | Time (min) | Pretreatment by ultrasonic probe | Without pretreatment | |
1 | −1 (30) | −1 (0) | −1 (10) | 0.0 | 0.0 |
2 | +1 (80) | −1 (0) | −1 (10) | 9.4 | 0.0 |
3 | −1 (30) | +1 (100) | −1 (10) | 0.0 | 5.9 |
4 | +1 (80) | +1 (100) | −1 (10) | 0.0 | 0.0 |
5 | −1 (30) | −1 (0) | +1 (40) | 0.0 | 0.0 |
6 | +1 (80) | −1 (0) | +1 (40) | 30.2 | 0.0 |
7 | −1 (30) | +1 (100) | +1 (40) | 0.0 | 0.0 |
8 | +1 (80) | +1 (100) | +1 (40) | 0.0 | 0.0 |
9 | 0 (55) | 0 (50) | 0 (25) | 0.0 | 0.0 |
10 | 0 (55) | 0 (50) | 0 (25) | 0.0 | 0.0 |
11 | 0 (55) | 0 (50) | 0 (25) | 0.0 | 0.0 |
12 | 0 (55) | 0 (50) | 0 (25) | 0.0 | 0.0 |
Enzymatic extract obtained after FS without exposure to reaction systems | 79.5 U mL−1 | 118.5 U mL−1 |