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Table 4 Experimental planning matrix (real and coded values) and relative activity results (%) for the conditions evaluated in the reaction system ultrasonic bath with enzymatic extracts produced from swine hair pretreated and non-pretreated with an ultrasonic probe

From: Reactional ultrasonic systems and microwave irradiation for pretreatment of agro-industrial waste to increase enzymatic activity

Assays Ultrasonic Bath Relative activity (%)
Temperature (°C) Power (%) Time (min) Pretreatment by ultrasonic probe Without pretreatment
1 −1 (30) −1 (0) −1 (10) 0.0 0.0
2 +1 (80) −1 (0) −1 (10) 9.4 0.0
3 −1 (30) +1 (100) −1 (10) 0.0 5.9
4 +1 (80) +1 (100) −1 (10) 0.0 0.0
5 −1 (30) −1 (0) +1 (40) 0.0 0.0
6 +1 (80) −1 (0) +1 (40) 30.2 0.0
7 −1 (30) +1 (100) +1 (40) 0.0 0.0
8 +1 (80) +1 (100) +1 (40) 0.0 0.0
9 0 (55) 0 (50) 0 (25) 0.0 0.0
10 0 (55) 0 (50) 0 (25) 0.0 0.0
11 0 (55) 0 (50) 0 (25) 0.0 0.0
12 0 (55) 0 (50) 0 (25) 0.0 0.0
Enzymatic extract obtained after FS without exposure to reaction systems 79.5 U mL−1 118.5 U mL−1