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Table 4 Experimental planning matrix (real and coded values) and relative activity results (%) for the conditions evaluated in the reaction system ultrasonic bath with enzymatic extracts produced from swine hair pretreated and non-pretreated with an ultrasonic probe

From: Reactional ultrasonic systems and microwave irradiation for pretreatment of agro-industrial waste to increase enzymatic activity

Assays

Ultrasonic Bath

Relative activity (%)

Temperature (°C)

Power (%)

Time (min)

Pretreatment by ultrasonic probe

Without pretreatment

1

−1 (30)

−1 (0)

−1 (10)

0.0

0.0

2

+1 (80)

−1 (0)

−1 (10)

9.4

0.0

3

−1 (30)

+1 (100)

−1 (10)

0.0

5.9

4

+1 (80)

+1 (100)

−1 (10)

0.0

0.0

5

−1 (30)

−1 (0)

+1 (40)

0.0

0.0

6

+1 (80)

−1 (0)

+1 (40)

30.2

0.0

7

−1 (30)

+1 (100)

+1 (40)

0.0

0.0

8

+1 (80)

+1 (100)

+1 (40)

0.0

0.0

9

0 (55)

0 (50)

0 (25)

0.0

0.0

10

0 (55)

0 (50)

0 (25)

0.0

0.0

11

0 (55)

0 (50)

0 (25)

0.0

0.0

12

0 (55)

0 (50)

0 (25)

0.0

0.0

Enzymatic extract obtained after FS without exposure to reaction systems

79.5 U mL−1

118.5 U mL−1