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Fig. 3 | Bioresources and Bioprocessing

Fig. 3

From: Development of chromosome-based T7 RNA polymerase and orthogonal T7 promoter circuit in Escherichia coli W3110 as a cell factory

Fig. 3

Comparison of sfGFP synthesis in the designed T7RNAP-carrying strains, with BL21(DE3) as a reference. sfGFP expressed from pET28a(+)-sfGFP plasmid (a) and pSU-T7-sfGFP plasmid (b). The fluorescence was analyzed by a microreader at 12 h, with excitation and emission wavelengths of 480 and 510 nm, respectively. The specific fluorescence for pET28a(+)-sfGFP and pSU-T7-sfGFP were presented in c and d, respectively. (+) denotes IPTG induction and (−) denotes no IPTG induction

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