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Bioresources and Bioprocessing
Fig. 5 | Bioresources and Bioprocessing

Fig. 5

From: Development of chromosome-based T7 RNA polymerase and orthogonal T7 promoter circuit in Escherichia coli W3110 as a cell factory

Fig. 5

SDS-PAGE analysis of CadA protein expression from pET28a (+)-CadA plasmid (a) with 0, 10–1, 10–2, 10–3, 10–4 to 10–5 mM IPTG for the designed T7RNAP carrier strains. Results are shown for BL21(DE3) (b), W3110::IL5 (c), W3110::L5 (d), and W3110::pI (e). CadA protein expressed from pSU-T7-CadA plasmid (f). Whole cell protein expression of CadA from pSU-T7-CadA in different strains with or without 0.1 mM IPTG was analyzed by 8% (w/v) SDS-PAGE (g). CadA has a size of 78.76 kDa, as indicated by the black arrow. (+) denotes IPTG induction and (−) denotes no IPTG induction

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