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Table 3 Quantification of relative protein of LacZ and T7RNAP by IPTG induction in BL21(DE3), W3110, W3110::IL5, W3110::L5, W3110::pI

From: Development of chromosome-based T7 RNA polymerase and orthogonal T7 promoter circuit in Escherichia coli W3110 as a cell factory

Relative protein amounts

BL21(DE3)*

W3110

W3110::IL5

W3110::L5

W3110::pI

LacZ

1.0

2.63

3.41

5.21

4.61

T7RNAP

1.0

8.72

6.72

11.92

  1. *LacZ and T7RNAP in BL21(DE3) with IPTG is defined as the reference of W derivatives