Table 4 Biomass, lysine consumption, DAP titer, DAP productivity, yield of in vivo time-course with pET28a(+)-CadA plasmid and 50 g/L lysine in BL21(DE3), W3110::IL5, W3110::L5 and W3110::pI, respectively
Strain* | Time (h) | Lysine consumption (g/L) | DAP titer (g/L) | Productivity (g DAP/L/h) | Yield (g/g, %) |
|---|---|---|---|---|---|
BL21(DE3) (+) | 6 | 9.07 ± 1.5 | 2.5 ± 0.1 | 0.42 ± 0.07 | 6.78 ± 1.17 |
12 | 9.40 ± 1.7 | 5.2 ± 1.0 | 0.43 ± 0.08 | 13.80 ± 2.50 | |
24 | 21.60 ± 1.4 | 11.3 ± 1.3 | 0.47 ± 0.06 | 30.05 ± 3.95 | |
W3110::IL5 (+) | 6 | 9.14 ± 2.4 | 4.0 ± 1.5 | 0.67 ± 0.25 | 11.59 ± 2.09 |
12 | 16.67 ± 3.7 | 10.4 ± 1.9 | 0.87 ± 0.16 | 29.38 ± 1.80 | |
24 | 18.13 ± 0.3 | 13.6 ± 1.6 | 0.57 ± 0.07 | 38.12 ± 5.89 | |
W3110::L5 (+) | 6 | 17.13 ± 1.8 | 11.8 ± 0.1 | 1.97 ± 0.02 | 33.62 ± 4.45 |
12 | 40.90 ± 2.8 | 29.0 ± 2.0 | 2.42 ± 0.16 | 82.46 ± 7.62 | |
24 | 45.01 ± 1.2 | 32.2 ± 0.2 | 1.34 ± 0.01 | 91.73 ± 8.07 | |
W3110::pI (+) | 6 | 14.50 ± 3.7 | 10.12 ± 1.1 | 1.69 ± 0.18 | 29.05 ± 2.51 |
12 | 28.45 ± 3.0 | 19.85 ± 2.1 | 1.65 ± 0.17 | 56.96 ± 3.05 | |
24 | 32.41 ± 2.0 | 22.61 ± 2.4 | 0.94 ± 0.10 | 64.89 ± 1.01 |