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Fig. 2 | Bioresources and Bioprocessing

Fig. 2

From: Development and fabrication of disease resistance protein in recombinant Escherichia coli

Fig. 2

a Gel electrophoresis analysis of DRP sequence by colony PCR. The N1 and N2 was the negative control by E. coli and pET21a(+) plasmid in the PCR reaction. The P indicates the positive control with DNA synthesis of DRP as a template, while numbers 1–4 represent each colony from pET21a-DRP in E. coli. M is the molecular weight of DNA marker. The targeted size is 832 bp. b DRP and DRP co-expression with pRARE vector for rare-codon optimization by SDS-PAGE analysis. S and WC represent the proteins from soluble and whole cell. The red arrows indicate the size of DRP

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