Fig. 2From: Development and fabrication of disease resistance protein in recombinant Escherichia colia Gel electrophoresis analysis of DRP sequence by colony PCR. The N1 and N2 was the negative control by E. coli and pET21a(+) plasmid in the PCR reaction. The P indicates the positive control with DNA synthesis of DRP as a template, while numbers 1–4 represent each colony from pET21a-DRP in E. coli. M is the molecular weight of DNA marker. The targeted size is 832 bp. b DRP and DRP co-expression with pRARE vector for rare-codon optimization by SDS-PAGE analysis. S and WC represent the proteins from soluble and whole cell. The red arrows indicate the size of DRPBack to article page