Table 1 Strains, plasmids and primers used in this study
From: Development and fabrication of disease resistance protein in recombinant Escherichia coli
Name | Description | Remark |
|---|---|---|
E. coli strains | ||
 DH5α | F-, Δ(argF-lac)169, φ80dlacZ58(M15), ΔphoA8, glnX44(AS), λ-, deoR481, rfbC1, gyrA96(NalR), recA1, endA1, thiE1, hsdR17 | Molecular cloning |
 BL21(DE3) | F– ompT gal dcm lon hsdSB(r –B m –B ) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) | T7-based expression |
Plasmids | ||
 pET21a | 3802 bp, KmR, RSF ori, T7 promoter, T7 terminator, LacI | This study |
 pET21a-DRP | 2922 bp, CmR, pUC ori, PlacI promoter, B0034 RBS, sfGFP | This study |
 pRARE | 2935 bp, CmR, pUC ori, PI promoter, B0034 RBS, sfGFP | Tegel et al. 2010 |
Primers | Â | Â |
 NdeI-DRP-F | GCCATATGTTGAAGGACGAATTGC | This study |
 XhoI-DRP-R | TGCTCGAGTCATTAATGGTGGTGATGGTGGTGGCTACCTCTTGG | This study |