Fig. 3
From: Highly efficient production of an influenza H9N2 vaccine using MDCK suspension cells
Optimization of infection parameters for H9N2 production in Xeno serum-free medium in shake flasks (n = 3). HA titers were shown in different panels when cells were infected at cell concentration of 6 × 106 cells/mL or 10 × 106 cells/mL with or without the medium exchange at TOI with a MOI of 10−2 (black circle), 10−3 (white square) and 10−4 (black triangle). a Cell concentration of 6 × 106 cells/mL without medium exchange. b Cell concentration of 6 × 106 cells/mL with medium exchange. c Cell concentration of 10 × 106 cells/mL without medium exchange. d Cell concentration of 10 × 106 cells/mL with medium exchange