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Fig. 5 | Bioresources and Bioprocessing

Fig. 5

From: Chitopentaose inhibits hepatocellular carcinoma by inducing mitochondrial mediated apoptosis and suppressing protective autophagy

Fig. 5

(GlcN)5 increased autophagy markers. A Representative image of TEM. Scale bar represents 2 μm. Autophagosomes per cell were counted and quantified. B Immunofluorescence results for LC3 puncta detection. Scale bar represents 50 μm. LC3 puncta per cell were counted and quantified. C Western blot analysis for p62, Beclin1, and LC3B. Cells were treated with different concentration of (GlcN)5. Cells were treated with or without (GlcN)5 (3.5 mM). Data were represented as mean ± SD Significance between groups was marked by various letters, a, b, c, etc. (One-way ANOVA). **, p < 0.01, ***, p < 0.001 (t-test)

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