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Bioresources and Bioprocessing
Fig. 6 | Bioresources and Bioprocessing

Fig. 6

From: Chitopentaose inhibits hepatocellular carcinoma by inducing mitochondrial mediated apoptosis and suppressing protective autophagy

Fig. 6

(GlcN)5 suppressed autophagy flux. A TEM results. Scale bar represents 2 μm. Autophagosomes per cell were counted and quantified. B Western blot analysis for p62 and LC3B. C LC3 puncta detection through transfection of mRFP-GFP-LC3. Scale bar represents 50 μm. LC3 puncta per cell were counted and quantified. D Colocalization analysis for autophagosomes and lysosomes. Scale bar represents 50 μm. Cells were treated with or without (GlcN)5 (5 mM), CQ (5 μM), or 3-MA (5 mM). Data were represented as mean ± SD Significance between groups was marked by various letters, a, b, c, etc. (One-way ANOVA)

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