Table 1 Box–Behnken design matrix for Bacillus drentensis strain S1 degrading APAP
Run no. | A:(pH) | B:temperature (°C) | C:concentration of Acetaminophen (mg/L) | D:agitation speed (rpm) | % Degradation of APAP | |
|---|---|---|---|---|---|---|
Experimental results | Predicted response | |||||
1 | 7 | 20 | 100 | 165 | 45 | 50.17 |
2 | 7 | 20 | 300 | 250 | 48 | 47.46 |
3 | 7 | 20 | 300 | 80 | 43 | 42.83 |
4 | 7 | 40 | 300 | 165 | 85 | 89.2 |
5 | 5 | 60 | 300 | 165 | 32 | 23.21 |
6 | 5 | 40 | 300 | 80 | 38 | 44.67 |
7 | 5 | 40 | 300 | 250 | 40 | 46 |
8 | 5 | 40 | 500 | 165 | 34 | 41.79 |
9 | 5 | 40 | 100 | 165 | 60 | 57.96 |
10 | 7 | 60 | 300 | 250 | 52 | 55.63 |
11 | 9 | 40 | 300 | 80 | 36 | 33 |
12 | 9 | 40 | 100 | 165 | 28 | 36.29 |
13 | 7 | 60 | 100 | 165 | 62 | 57.04 |
14 | 7 | 40 | 300 | 165 | 88 | 89.2 |
15 | 7 | 60 | 300 | 80 | 37 | 43.29 |
16 | 9 | 20 | 300 | 165 | 32 | 32.04 |
17 | 7 | 60 | 500 | 165 | 45 | 42.83 |
18 | 7 | 40 | 300 | 165 | 89 | 89.2 |
19 | 7 | 40 | 300 | 165 | 93 | 89.2 |
20 | 7 | 40 | 500 | 250 | 62 | 58.33 |
21 | 7 | 20 | 500 | 165 | 38 | 33.38 |
22 | 7 | 40 | 500 | 80 | 57 | 52.21 |
23 | 7 | 40 | 300 | 165 | 91 | 89.2 |
24 | 9 | 40 | 300 | 250 | 43 | 45.13 |
25 | 9 | 40 | 500 | 165 | 34 | 31.46 |
26 | 7 | 40 | 100 | 250 | 74 | 70.04 |
27 | 9 | 60 | 300 | 165 | 35 | 30.88 |
28 | 5 | 20 | 300 | 165 | 38 | 41.17 |
29 | 7 | 40 | 100 | 80 | 64 | 60.21 |