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Bioresources and Bioprocessing
Fig. 2 | Bioresources and Bioprocessing

Fig. 2

From: Enhanced plumbagin production in Plumbago indica root culture by simultaneous and sequential dual elicitations using chitosan with ʟ-alanine and methyl-β-cyclodextrin

Fig. 2

Effect of sequential treatments using chitosan followed by other elicitors on a plumbagin content; b total plumbagin yield; c dry root biomass. Twelve-day P. indica root cultures were treated with 150 mg L−1 chitosan followed by 14-day treatments with methyl jasmonate, salicylic acid, 1-naphthol, yeast extract, MCD, AgNO3, sodium acetate and ʟ-alanine. Control is untreated root cultures with equal amount of distilled water or 2% ethanol added. Chitosan is the singly treated at 14 days. Measurements were taken after 72-h treatment from day 14 treatments. Values are mean ± SE of triplicate experiments. Data were analyzed by one-way ANOVA, using Tukey’s test for multiple comparison. Different superscripts letters between the groups are significantly different at p < 0.05. MCD; methyl-β-cyclodextrin

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