Fig. 2

a Genotype verification primers. PCR product would be 650 bp by the primers 0101C1 and 5DC3 when genomic DNA of WT-Δ0101 was used as the template while WT was 0 bp. PCR product would be 470 bp by the primers 5DCF and 0101C2 when genomic DNA of WT-Δ0101 was used as the template while WT was 0 bp. PCR product would be 1250 bp by the primers 0101C1 and 0101C2 when genomic DNA of WT-Δ0101 was used as the template while WT was 1120 bp. b Genotype verification for SACE_0101 gene inactivation in mutant strain WT-Δ0101. Lane 1: PCR product (650 bp) by the primers 0101C1 and 5DC3 when genomic DNA of WT-Δ0101 was used as the template. Lane 2: PCR product (0 bp) by the primers 0101C1 and 5DC3 when genomic DNA of wild type (WT) was used as the template. Lane 3: PCR product (470 bp) by the primers 5DCF and 0101C2 when genomic DNA of WT-Δ0101 was used as the template. Lane 4: PCR product (0 bp) by the primers 5DCF and 0101C2 when genomic DNA of WT was used as the template. Lane 5: PCR product (1250 bp) by the primers 0101C1 and 0101C2 when genomic DNA of WT-Δ0101 was used as the template. Lane 6: PCR product (1120 bp) by the primers 0101C1 and 0101C2 when genomic DNA of WT was used as the template. Lane 7: DL 2000 marker. Lane 8: PCR product (760 bp) by the primers neoF and neoR when genomic DNA of WT-Δ0101 was used as the template. Lane 9: PCR product (0 bp) by the primers neoF and neoR when genomic DNA of WT was used as the template