Skip to main content

Table 2 Primers used in this study

From: A two-component system gene SACE_0101 regulates copper homeostasis in Saccharopolyspora erythraea

Primers

Sequence (5′–3′)

PCR amplification

 0101UF

ACGGCCAGTGCCAAGCTTTTGAGCTCCTGGTGCTTCTTCA

 0101UR

TGCGGCAGCGTGAAGCTTATCAGGATCTTGCTCAAGAGTC

 0101DF

ATGCGCGATATCGAATTCAAGATTGGCAGCACACGCAT

 0101DR

GGACATGATTACGAATTCTCGATGCCGATGTCGTAGCT

 0101C1

GAGAAGATCTTCGAACGCTTCTC

 0101C2

AGATACGGCTGTCGGCGAAC

 5DCF

ATGAGCAGTTCCTGGGCTTTC

 5DC3

CCGATTGTCTGTTGTGCCCAGTC

RT-PCR

 16srRNA-F

AGATACCCTGGTAGTCCACG

 16srRNA-R

CGTTGCGCTTCTTGGAATGG

 0528RT-F

AGCTGGGTGTCACCGAGTTC

 0528RT-R

AGCGAGACGGCGTGGTTCCA

 1321RT-F

GTCATCGGGCAGAACTTCTAC

 1321RT-R

CCAGCATGACCACGATCAG

 2475RT-F

CTGGATGCCTACTTCGACAAG

 2475RT-R

ACCCGACCTTGTTCTTGC

  1. Restriction sites are underlined (UF/UR: HindIII, DF/DR: EcoRI)