Efficacy of alcohol/sugar aqueous biphasic system on partition of bovine serum albumin

Bioresources and Bioprocessing

Table 1 Effect of types and concentration of phase-forming components on the partition efficiency of BSA with propanol/sugar ABS

Types of ABS	Concentration of sugar, % (w/w)	Concentration of alcohol, % (w/w)	K	Recovery yield, %
1-Propanol/Maltose	22	35	6.03 ± 0.17	87.84 ± 0.61
	23	37	4.40 ± 0.43	81.70 ± 1.88
	24	38	2.57 ± 0.46	72.58 ± 3.58
	25	39	1.74 ± 0.14	64.39 ± 1.81
	26	39	0.90 ± 0.25	49.40 ± 4.79
1-Propanol/Sucrose	26	33	1.17 ± 0.07	63.61 ± 1.48
	27	34	1.26 ± 0.11	56.71 ± 2.11
	28	35	1.36 ± 0.15	62.86 ± 2.64
	29	36	0.93 ± 0.10	48.21 ± 2.72
	30	37	0.72 ± 0.08	42.33 ± 3.32
1-Propanol/Glucose	24	36	1.14 ± 0.08	50.42 ± 2.10
	25	38	1.36 ± 0.05	58.11 ± 1.48
	26	39	1.01 ± 0.08	50.76 ± 2.58
	27	40	0.59 ± 0.02	38.04 ± 0.71
	28	41	0.38 ± 0.09	30.75 ± 4.91
2-Propanol/Maltose	31	32	2.41 ± 0.39	70.51 ± 3.35
	32	33	1.19 ± 0.19	54.19 ± 3.88
	33	34	0.85 ± 0.03	42.10 ± 0.92
	34	35	0.76 ± 0.14	43.06 ± 4.63
	35	36	0.33 ± 0.03	23.38 ± 1.66
2-Propanol/Sucrose	33	38	1.01 ± 0.03	48.13 ± 0.84
	34	39	0.75 ± 0.02	43.02 ± 0.60
	35	40	0.74 ± 0.04	42.46 ± 1.19
	36	41	0.22 ± 0.05	17.71 ± 3.15
	37	41.5	0.08 ± 0.03	7.42 ± 2.90
2-Propanol/Glucose	31	39	0.82 ± 0.07	44.95 ± 2.00
	32	40	0.59 ± 0.00	39.84 ± 0.10
	33	41	0.35 ± 0.05	27.38 ± 2.70
	34	42	0.32 ± 0.04	25.82 ± 2.51
	35	43	0.48 ± 0.02	34.33 ± 1.10

Alcohol/sugar ABSs comprised of different phase-forming component concentration were selected for investigation based on their relative position in the biphasic region which gave a V_R of 1.0 at equilibrium