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Bioresources and Bioprocessing

Table 2 The actual, predicted, and residual responses for each experimental run using the Box–Behnken design for high cell density production

From: Optimization of cultivation medium and cyclic fed-batch fermentation strategy for enhanced polyhydroxyalkanoate production by Bacillus thuringiensis using a glucose-rich hydrolyzate

Experimental run

Variables

Cell density (OD600)

Yeast extract (g L−1)

Hydrolyzate (% v/v)

Time (h)

pH

Actual

Predicted

Residual

1

6

50

24

7.2

2.0359

2.0517

− 0.0158

2

10

50

24

7.2

2.1195

2.0293

0.0902

3

6

100

24

7.2

1.3056

1.4088

− 0.1032

4

10

100

24

7.2

1.7119

1.7091

0.0028

5

8

75

18

7.0

1.6736

1.5987

0.0749

6

8

75

30

7.0

1.9265

1.9670

− 0.0405

7

8

75

18

7.4

1.6336

1.6061

0.0275

8

8

75

30

7.4

1.8854

1.9733

− 0.0879

9

8

75

24

7.2

2.2129

2.1609

0.0519

10

6

75

24

7.0

1.8923

1.8377

0.0546

11

10

75

24

7.0

1.8094

1.8706

− 0.0613

12

6

75

24

7.4

1.8057

1.7384

0.0672

13

10

75

24

7.4

1.9349

1.9835

− 0.0486

14

8

50

18

7.2

1.7275

1.7876

− 0.0601

15

8

100

18

7.2

1.2236

1.1973

0.0262

16

8

50

30

7.2

2.0264

2.0466

− 0.0203

17

8

100

30

7.2

1.7399

1.6739

0.0660

18

8

75

24

7.2

2.1112

2.1349

− 0.0238

19

6

75

18

7.2

1.7387

1.7779

− 0.0392

20

10

75

18

7.2

1.8382

1.8676

− 0.0294

21

6

75

30

7.2

2.1328

2.09634

0.0364

22

10

75

30

7.2

2.3309

2.2847

0.0463

23

8

50

24

7.0

2.1408

2.1552

− 0.0144

24

8

100

24

7.0

1.2805

1.2938

− 0.0133

25

8

50

24

7.4

1.8025

1.7821

0.0203

26

8

100

24

7.4

1.7019

1.6804

0.0214

27

8

75

24

7.2

2.2071

2.2352

− 0.0281

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