Fig. 6From: Redirection of metabolic flux in Shewanella oneidensis MR-1 by CRISPRi and modular design for 5-aminolevulinic acid productionCharacterization of glucose-consuming strains in different minimal media. MR-1 cells with medium and low copy numbers for glk-galP were compared with the wild-type strain, and the specific growth rates (a) and glucose consumption (b) were analyzed. ALAS and GroELS were co-expressed with glk-galP as two plasmids. c Protein expression was observed by SDS-PAGE at 12Â h, which ALAS and GroEL at sizes of approximately 48Â kDa and 63Â kDa, respectively. S soluble protein, P insoluble protein, M protein ladder marker. d ALA production and biomass in terms by OD600 were analyzed at 12Â h and 24Â hBack to article page