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Fig. 6 | Bioresources and Bioprocessing

Fig. 6

From: Redirection of metabolic flux in Shewanella oneidensis MR-1 by CRISPRi and modular design for 5-aminolevulinic acid production

Fig. 6

Characterization of glucose-consuming strains in different minimal media. MR-1 cells with medium and low copy numbers for glk-galP were compared with the wild-type strain, and the specific growth rates (a) and glucose consumption (b) were analyzed. ALAS and GroELS were co-expressed with glk-galP as two plasmids. c Protein expression was observed by SDS-PAGE at 12 h, which ALAS and GroEL at sizes of approximately 48 kDa and 63 kDa, respectively. S soluble protein, P insoluble protein, M protein ladder marker. d ALA production and biomass in terms by OD600 were analyzed at 12 h and 24 h

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