Table 1 Strains used in this study
Strains | Description | Source |
|---|---|---|
E. coli DH5α | Cloning strain, F–endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG purB20 φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK–mK+), λ– | Lab stock |
E. coli WM3064 | Donor strain for conjugation, thrB1004 pro thi rpsL hsdS lacZΔM15 RP4-1360 Δ(araBAD)567 ΔdapA1341::[erm pir] | Lab stock |
S. oneidensis MR-1 | Wild type strain | Lab stock |
MR1::T7R | Integration of T7 RNA polymerase onto wild type S. oneidensis MR-1 genome | Yi and Ng (2020) |
RcAG | MR1::T7R harboring plasmid of RcA-EcG | This study |
RcAG-pta | MR1::T7R harboring pSUKM-RcA-EcG and pSM-dCas9-pta | This study |
RcAG-ackA | MR1::T7R harboring pSUKM-RcA-EcG and pSM-dCas9-ackA | This study |
15Agg | MR1::T7R harboring pYCI-15A-gg | Â |
322gg | MR1::T7R harboring pYCI-gg | This study |
RcAG-322gg | MR1::T7R harboring pSUM-RcA-EcG and pYCI-322-gg | This study |
RcAG-15Agg | MR1::T7R harboring pSUM-RcA-EcG and pYCI-15A-gg | This study |
M::TRG | MR1::T7R with integration of RchemA and EcgroELS under T7 promoter | This study |