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Table 3 Purification techniques documented in literature for recovery of xylooligosaccharides (XOs)

From: Xylanolytic Bacillus species for xylooligosaccharides production: a critical review

Product

Purification techniques

Substrate

pH and temperature

Recovery % (DP*)

Reference

Acidic XOs

Anion-exchange and size-exclusion chromatography

Birchwood xylan

50  °C

85 (NA)

(Christakopoulos et al. 2003)

Xylobiose and xylotriose

Nanofiltration

Corncob meal

pH 5.5, 55  °C

74.5 (< 5)

(Yuan et al. 2004)

XOs

Gel filtration chromatography

Oil palm empty fruit bunch

NA

83–85 (5–40)

(Ho et al. 2014)

XOs

Ultrafiltration

Almond shells

179 °C

58.3 (NA)

(Nabarlatz et al. 2007)

Xylobiose

Nanofiltration

Empty fruit bunch

pH 5,50  °C

90.1 (NA)

(Wijaya et al. 2020)

XOs

Ultrafiltration

Wheat bran

pH 6.5, 60  °C

44.4 (2–5)

(Geetha and Gunasekaran 2017)

Xylohexose Xylobiose and xylotriose

High-performance anion exchange

Chemical pulp

pH 5,50  °C

47 & 90.5 (NA)

(Wang et al. 2018)

XOs

HPLC

Corncob

pH 8, 50  °C

32.5 (≤ 4)

(Lin et al. 2011)

  1. DP* degree of polymerization, NA not available