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Bioresources and Bioprocessing
Fig. 1 | Bioresources and Bioprocessing

Fig. 1

From: Tuning a bi-enzymatic cascade reaction in Escherichia coli to facilitate NADPH regeneration for ε-caprolactone production

Fig. 1

Bi-enzyme catalysis for ε-caprolactone production from cyclohexanol. a The schematic of a cascade reaction based on whole-cell biocatalysis system. Cyclohexanol is enzymatically converted into cyclohexanone by alcohol dehydrogenase (ADH) with NADPH generation, which is oxidized into ε-caprolactone by cyclohexanone monooxygenase (CHMO) with NADPH consumption. b Expression vector constructions to regulate the protein expression through RBS sequence replacements

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