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Bioresources and Bioprocessing
Fig. 2 | Bioresources and Bioprocessing

Fig. 2

From: Tuning a bi-enzymatic cascade reaction in Escherichia coli to facilitate NADPH regeneration for ε-caprolactone production

Fig. 2

Expression levels of E. coli BL21(DE3) containing alcohol dehydrogenase (ADH) and cyclohexanone monooxygenase (CHMO) in the dual promoter vectors. a SDS-PAGE electropherogram. 10 OD600 Cells were collected by centrifugation and resuspended in 1 mL lysis buffer and subjected to sonication for cell lysis. S = supernatant fractions of cell lysates, P = precipitates of cell lysates, M = standard protein marker. The arrows indicate CHMO (62 kDa) and ADH (28 kDa), respectively. b Enzyme activity (U/mL) of ADH and CHMO for the four recombinant E. coli strains. Measurements were performed in three independent experiments

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