Tuning a bi-enzymatic cascade reaction in Escherichia coli to facilitate NADPH regeneration for ε-caprolactone production

Bioresources and Bioprocessing

Table 1 Production of ε-caprolactone in different engineering host strains

Cells		Substrate		ε-caprolactone	Reaction	Yield (mol/mol substrate/cell mass)	References
Type	Mass (g/L)	Type	Concentration (mM)	Concentration (mM)	Reaction	Yield (mol/mol substrate/cell mass)	References
Pseudomonas taiwanensis	6.8^a	Cyclohexane	200	17.0	Single batch	0.0125 ^a	(Karande et al. 2018)
Geotrichum candidum	100	Cyclohexanol	10	10	Single batch	0.0100	(Silva et al. 2017)
Geotrichum candidum	100	Cyclohexanol	60	58.4	Six batches	0.0097	(Silva et al. 2017)
E. coli	10	Cyclohexanol	60 100	36^c 28^c	Single batch	0.0600 0.0280	(Kohl et al. 2018)
E. coli	100	Cyclohexanol ^b	60	57^c	Single batch	0.0095	(Schmidt et al. 2015b)
E. coli	100	Cyclohexanol ^b	80	35^c	Single batch	0.0044	(Schmidt et al. 2015b)
E. coli	35^a	Cyclohexanone	245^c	134	Four batches	0.0156 ^a	(Lee et al. 2007)
E. coli	10	Cyclohexanol	60	47.7	Single batch	0.0795	This study
			80	50.5	Single batch	0.0631
			160	126.0	Four batches	0.0788

^aDry cell mass concentration
^bReaction mixtures were supplemented with equimolar amount of D-glucose and/or acetone for cofactor recycling
^cCalculated from figures in the literature