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Fig. 1  | Bioresources and Bioprocessing

Fig. 1 

From: One-pot production of butyl butyrate from glucose using a cognate “diamond-shaped” E. coli consortium

Fig. 1 

Construction of a “diamond-shaped” consortium for the direct production of butyl butyrate from glucose using two E. coli strains with the same genetic background. The butyric acid-producing strain is shown in yellow, and the butanol-producing strain in blue. A shared pathway from glucose to butyryl-CoA is shown in light grey in overlap between the two strains. A green arrow indicates the thioesterase pathway (encoded by yciA, yciAh or tesB), while the ptb-buk and ptb-buk2 route is indicated by a grey arrow. The production routes of precursors (butanol and butyric acid) and their conversion into butyl butyrate with the assistance of lipase enzyme forms a “diamond-shaped” structure, which was used to define the consortium. The genes hbd (3-hydroxybutyryl-CoA dehydrogenase), crt (crotonase), adhE2 (aldehyde/alcohol dehydrogenase), ptb (phosphate butyryltransferase), as well as buk and buk2 (butyrate kinase) are derived from C. acetobutylicum; the ter gene (trans-enoyl-CoA reductase) is from Treponema denticola; yciA and tesB (acyl-CoA thioesterase) are from E. coli; yciAh (acyl-CoA thioesterase) is from Haemophilus influenza

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