Table 1 Strains and plasmids used in this study
Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
Strains | ||
E. coli EB243 | Derived from BW25113; Containing butanol synthesis pathway genes (atoB, hbd, crt, ter, adhE2) and fdh, as well as deletions of adhE, eutE, yqhD, ackA, pta, hyc-hyp, fdhF, poxB, pck, fumB, fumAC, tdcD, mdh, focA, ppc, mgsA, yieP, stpA, yqeG, and yagM | (Dong et al. 2017) |
E. coli EB243ΔadhE2 | EB243 derivative, with adhE2 deleted | This study |
E. coli EB243ΔadhE2-pAC2 | EB243 derivative, harboring the plasmid pAC2 | This study |
E. coli EB243ΔadhE2-pAC2-ptb-buk | EB243 derivative, harboring the plasmid pAC2-ptb-buk | This study |
E. coli EB243ΔadhE2-pAC2-ptb-buk2 | EB243 derivative, harboring the plasmid pAC2-ptb-buk2 | This study |
E. coli EB243ΔadhE2-pAC2-yciAh | EB243 derivative, harboring the plasmidpAC2-yciAh | This study |
E. coli EB243ΔadhE2-pAC2-tesB | EB243 derivative, harboring the plasmid pAC2-tesB | This study |
E. coli EB243ΔadhE2-pAC2-yciA | EB243 derivative, harboring the plasmid pAC2-yciA | This study |
E. coli EB243ΔadhE2::yciAh | Derived from EB243ΔadhE2; with the synthetic thioesterase gene “yciAh” integrated into the chromosome | This study |
Plasmids | ||
pAC2 | pACYC184 derivative, miniPtac, cat, KanR | (Zhao et al. 2019) |
pAC2-ptb-buk | pAC2 derivative, expressing the ptb-buk genes | This study |
pAC2-ptb-buk2 | pAC2 derivative, expressing the ptb-buk2 genes | This study |
pAC2-yciAh | pAC2 derivative, expressing the yciAh gene | This study |
pAC2-yciA | pAC2 derivative, expressing the yciA gene | This study |
pAC2-tesB | pAC2 derivative, expressing the tesB gene | This study |
pTargetF | aadA, guide RNA transcription | (Jiang et al. 2015) |
pCas | KanR, gam-bet-exo, cas9 | (Jiang et al. 2015) |
pTargetF-adhE2 | Derived from pTargetF, adhE2 knockout vector | This study |